Molecular cloning and characterization of a surface-localized adhesion protein in Mycoplasma bovis Hubei-1 strain.

Molecular cloning and characterization of a surface-localized adhesion protein in Mycoplasma bovis Hubei-1 strain.

Blog Article

Mycoplasma bovis (M.bovis) is an important pathogen that causes various bovine diseases, such as mastitis in cows and pneumonia in calves.The surface proteins are generally thought to play a central role in the pathogenesis of this organism.We screened the entire genome of M.

bovis Hubei-1 and discovered a gene named vpmaX read more that encodes the 25 kDa variable surface lipoprotein A (VpmaX).Sequence analysis revealed that VpmaX contains several repetitive units and a typical bacterial lipoprotein signal sequence.The vpmaX gene was cloned and expressed in E.coli to obtain recombinant VpmaX (rVpmaX).

Western blot analysis using a rabbit antibody against rVpmaX demonstrated that VpmaX is a membrane protein.Immunostaining visualized via confocal laser scanning microscopy showed that rVpmaX was able to adhere to embryonic bovine lung cells (EBL), read more and this was also confirmed by a sandwich ELISA.In summary, a surface-localized adhesion protein was identified in M.bovis Hubei-1.